eBIC: The UK National Cryo-EM Facility

The electron Bio-Imaging Centre was established at Diamond Light Source following the award of a £15.6 million grant from the Wellcome Trust, the Medical Research Council (MRC) and the Biotechnology and Biological Sciences Research Council (BBSRC).

eBIC mission:

• Provide 24/7 free user access through peer-review process
• Develop cutting-edge technology with in-house research program
• Grow and train cryo-EM user community
• Foster integrated structural biology within Diamond and beyond

eBIC is a CryoEM center providing scientists with state-of-the-art experimental equipment and expertise in the field of cryo-electron microscopy, for single particle analysis, electron tomography and electron diffraction. The location of eBIC enables scientists to combine their techniques with many of the other cutting-edge approaches that Diamond offers. 

Currently eBIC houses five Titan Krios microscopes, a Talos Arctica, two Glacios, a Scios and an Aquilos cryo-FIB/SEM, and a Leica CryoCLEM.

We now have an opportunity for a Postdoctoral Research Associate to join Professor Zhang’s lab at the Electron Bio-Imaging Centre (eBIC), Diamond Light Source, UK to develop new methods in cryoET and subtomogram averaging for in situ structural biology.

In situ structural biology by cryoET is a fast-growing field. However, software to push the quality and resolution of tomographic reconstruction is urgently needed. The current existing reconstruction software packages have limitations in refining all 3D spatial parameters, resulting suboptimal reconstruction. We have recently developed a new tilt series alignment algorithm for all 5 spatial parameters at each tilt, which is also performed entirely in Fourier space and vastly increases the processing speed.  We are seeking highly motivated scientist to develop this further, including new subtomogram averaging methods in conjunction with our new cryoET data collection schemes

Read more here...Postdoctoral Research Associate - Cryo EM/ET Software