Wellcome-MRC in situ Cryo-ET Workshop 2024

Oct 14. till Oct 18.

Wellcome-MRC in situ Cryo-ET Workshop 2024

eBIC (Diamond light source) has partnered with the Astbury Biostructure Lab (University of Leeds), the Scottish Centre for Macromolecular Imaging (University of Glasgow), Midlands Regional Cryo-EM Facility (University of Leicester) and Birkbeck college (University of London) to host training courses between 2021-2026 in cryo-EM. This training program would not be possible without these partners, and is funded by Wellcome and MRC (218785/Z/19/Z).

Date
14/10/20242024-10-14T09:00:00 - 18/10/20242024-10-18T17:00:00
Time
09:00 - 17:00
Location
In-person, Diamond Light Source (and online via zoom)

Overview 

This is a hybrid event targeted at researchers new to the field. The course will provide fundamental lectures, demonstrations and practicals in cellular sample preparation, FIB milling, lamella preparation, cryo-ET data collection, initial quality assessment and data processing.

Lectures will be held in-person at Diamond Light Source and will be broadcast to the wider community via Zoom. Demonstrations and hands-on practical sessions covering cellular sample preparation, correlative FIB milling, lamella preparation, cryo-ET data collection, initial quality assessment and data processing will be available to in-person attendees only.

Due to limited space, in-person places are strictly limited to 8 delegates. These spaces are funded through the Wellcome/MRC cryoEM training program (218785/Z/19/Z). We welcome applications from the UK, Europe and international applicants. Spaces will be allocated based on merit and anticipated benefit. It is expected that applicants will have a background in imaging, electron microscopy and structural biology, ideally with some experience with FIB and/or electron tomography. Also, we expect applicants to have the means to apply their newly acquired knowledge at their home institution (i.e., you have access to the relevant instruments at your home institute, have plans in place to acquire instrumentation or have access through a local institute). Once all applications have been received, these will be reviewed and successful candidates informed by the dates specified below (please see 'Key Dates' for full information).

For further information regarding how to apply for an in-person place, please see below.

There is no fee to attend this course in-person or online, but registration is mandatory. For the 8 Wellcome/MRC funded in-person places, accommodation and food will be provided. We offer financial support for travel to participants with limited access to funding. This support includes reimbursement of travel expenses up to £250. Any expenses exceeding these amounts will need to be covered by the participants.

Programme

Detailed programme will be available in due course.

Day 1: Monday 14th October 2024

  • Lectures covering an introduction to cryo-ET workflows, cellular sample preparation and sample vitrification (in-person and virtual)
  • Demonstration covering sample vitrification
  • Practical covering cryo-ET sample preparation and sample vitrification (in-person only)

Day 2: Tuesday 15th October 2024

  • Lectures covering correlative cryo-ET workflows, cryo-FIB-SEM and lamella preparation (in-person and virtual)
  • Practicals covering principles of cryo-FIB-SEM set up and introduction to lamella preparation (in-person only)

Day 3: Wednesday 16th October 2024

  • Practicals covering principles of cryo-FIB-SEM set up and introduction to lamella preparation (in-person only)

Day 4: Thursday 17th October 2024

  • Demonstration of cryo-ET screening and data collection (in-person and virtual)
  • Practical covering cryo-ET screening and data collection (in-person only)

Day 5: Friday 18th October 2024

  • Lectures covering cryo-ET image processing and reconstruction (in-person and virtual)
  • Practical sessions covering cryoET image processing (in-person only) 
  • Student learning & feedback discussion (in-person only)

How to Apply (in-person spaces)

APPLICATIONS FOR IN-PERSON SPACES ARE NOW CLOSED

There are 8 in-person spaces available to selected members of the community. Places will be allocated on the grounds of merit and anticipated benefit.

All applicants must meet the following criteria:

  • You have a background in imaging, electron microscopy and structural biology, ideally with some experience with FIB and/or electron tomography.
  • You have access to equipment in order to apply the newly acquired knowledge at your home institution (i.e., you have access to the relevant instruments at your home institute, have plans in place to acquire instrumentation or have access through a local institute).

Before beginning the application process, please prepare the following documents to upload to the application page:

  • A cover letter describing your background, motivation for applying for the course and how this will benefit your institute and your research.  Please also describe your current role/position, any access routes for instrumentation and your plans for using cryo-ET and FIB lamella preparation. 
  • A letter of support from your manager or supervisor.

If successful, you will be asked to formally accept your place and register for the event. Academic places will be free of charge and include:

  • all meals and refreshments
  • accommodation if required, checking in on Sunday 13th October and checking out on Friday 18th October
  • travel costs up to £250 for participants with limited access to funding (any expenses exceeding these amounts will need to be covered by the participants)

Key Dates

Applications for in-person attendance: 6th August - 31st August 2024

In-person candidate selection: All applicants will be notified with the outcome of their application by the 13th September 2024

Registration for online attendance: open from the 13th September - 4th October 2024

Contact

For any further information about this workshop, or if you are interested in sponsoring this event, please contact lorna.malone@diamond.ac.uk. 

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