Tel: +44 (0) 1235 56 7480
Email: EBICadmin@diamond.ac.uk
Office hours: 08.30-16.30(UK) Mon-Fri
Peijun Zhang
Tel: +44 (0) 1235 77 8878
Email: peijun.zhang@diamond.ac.uk
Daniel Clare
Tel: +44 (0) 1235 56 7501
Email: daniel.clare@diamond.ac.uk
Yuriy Chaban
Tel: +44 (0) 1235 77 8207
Email: yuriy.chaban@diamond.ac.uk
Christos Savva
Tel: +44 (0) 1235 77 8976
Email: christos.savva@diamond.ac.uk
Tel: +44 (0) 1235 39 4182
Email: lorna.malone@diamond.ac.uk
Email: martin.walsh@diamond.ac.uk
Tel: +44 (0)1235 778518
There is a wealth of information on cryo-EM in the community mailing lists of 3DEM and CCPEM which remain very active. Generally you may find answers to very specific technical and academic questions, as well as broader high-level discussions.
There are otherwise lots of resources on the internet that aim to provide training and education in cryo-EM. This page aims to collate some of the resources that are available to give a helpful starting point to newcomers and or refresher for experienced cryo-EM structural biologists alike. It is not intended to be an exhaustive reference list.
When using these resources, the reader is encouraged to consider when the resource was created. There is always something to be learned but cryo-EM techniques have and will continue to develop rapidly, so when you are at the microscope in your own institute do follow the advice and protocols that are in place locally.
Courses and Workshops at eBIC
We offer regular courses and workshops on cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) at eBIC, open to anyone interested in learning or applying these techniques. Recordings of lectures from previous events are available on our Courses, Workshops and Events page, with individual recordings accessible via each event’s dedicated page.
Below are links to our most recent events. Recordings from these sessions are available at the bottom of their respective event pages:
Wellcome-MRC Cryo-EM in Structural Biology 2025 – A general introduction to cryo-EM
Wellcome-MRC In Situ Cryo-ET Workshop 2024 – An introduction to cryo-ET
Demonstrations of EPU and TOMO software
The videos below feature our EM scientists demonstrating the setup of single particle and tomography data collection workflows at eBIC, using EPU and TOMO, respectively.
The following are a list of courses we are aware of that provide a comprehensive overview of Cryo-EM which may serve as a helpful overview for newcomers to the field and/or refresher for experienced cryo-EM structural biologists alike. This is not an exhaustive list but may serve as a helpful starting point.
Single Particle Analysis - An Overview
Cryo Electron Tomography - An Overview
Vitrification, Clipping and Loading (Animation)
Vitrification, Clipping and Loading (Technical Guide)
JoVE: Single Particle Cryo-Electron Microscopy: From Sample to Structure
General:
Quick tutorial: The Linux command line:
https://ubuntu.com/tutorials/command-line-for-beginners#1-overview
Course: General introduction & computational skills:
https://software-carpentry.org/
Linux, shell, bash:
Quick tutorial: Bash commands:
https://www.datacamp.com/tutorial/shell-commands-data-scientist
Cheat sheet: Linux command line:
https://cheatography.com/davechild/cheat-sheets/linux-command-line/
Cheat sheet: awk command:
https://cheatography.com/tme520/cheat-sheets/awk-english/
Course: Introduction to bash scripting:
https://www.datacamp.com/courses/introduction-to-bash-scripting
Python:
Cheat Sheet: Python & data science:
https://www.datacamp.com/cheat-sheet
Microscope locations worldwide
Google map
Thermo Fisher Scientific resources
Interactive cryo-EM technique overview
How to make a microscope (YouTube - suitable for the public)
Fundamental Tutorials in Transmission Electron Microscopy
http://rodenburg.org
Transmission Electron Microscope Technical Diagrams
https://www.globalsino.com/
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