Welcome to I04

8^th June 2020: Access to MX beamlines is currently operating under new guidelines. Please read carefully.

I04 variable and microfocus MX

I04 is one of seven macromolecular crystallography beamlines at Diamond. The typical working wavelength is 0.9537 Å (13.00 keV) but it is tuneable over the wavelength range 0.69 - 2.066 Å (6 keV - 18 keV). On request it is also possible to work at a wavelength as low as 0.62 Å (20keV)^.

The beamsize can be focussed from 10 x 5 µm²to 110 x 100 µm² (h x v) across the available energy range. On request smaller beams in the vertical direction are possible. This will primarily depend on the working wavelength.

All MX beamlines use SPINE standard pins and Unipucks from cryocooled samples. The beamline can be accessed remotely.

The beamline is now equipped with the first Eiger2 XE 16M detector which will enable faster data collection and new data collection protocols.

22/07/2019:

I03 and I04 are equipped with Eiger2 XE 16M detectors - collect at up to 560 Hz!

24/06/2019:

Beamline workstations have been upgraded to RedHat 7, more info here

16/01/2019:

Latest generation Eiger2 X 16M detector on I04. More information here.

16/11/2017:

Smargon and new goniometer positioners installed - fast grid scans available.

01/09/2017:

UK academic users book your DHL shipping to Diamond via ISPyB - with built in tracking.

20/04/2017:

We will be barcoding pucks from April 2017 onwards.

27/01/2017:

Data reprocessing now available in ISPyB. See here

25/04/2016:

New monochromator for I04. Big improvements, more to learn and deliver to users over coming months.

17/11/2015:

New remote access connection available

11/09/2015:

New BART robot installed on I04 - 37 pucks, 592 samples, 2.5x faster exchange times!

21/01/2015:

New focus options on I04. Variable beam size from 5 - 100 microns between 6 - 14 keV.

14/04/2014:

New web interface for ISPyB launched. For more information click here.

14/04/2014:

Go to GDA - Messages- Beamline notes to read the message of the day. Click here for more.

06/01/2014:

Loan pucks available upon request, check here

19/09/2013:

Test your network connection to Diamond with our SpeedTest

30/06/2013:

Crystal washer now available

15/04/2013:

I04 installed a Pilatus 6M-F detector in April 2013.

25/01/2013:

Remote access available on all MX beamlines at all times since January 2013

New insertion device in July 2022 increases flux x4 with a standard energy of 13 KeV

New BART robot - 37 pucks, 592 samples, 17 s exchange

The new three-axis SmarGon goniometer

The new Eiger2 XE 16M is available since January 2019

Automated Single Crystal Crystallography

Example of using I04 for structural characterization

<p><a title="Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092" href="https://www.jove.com/video/56294/biochemical-structural-characterization-carbohydrate-transport">Biochemical and Structural Characterization of the Carbohydrate Transport Substrate-binding-protein SP0092</a></p>

Current Status

: I04 flux profile for 30 x 20 micron (h x v) beam size

Item	Available
Microfocus Beam	✓
Variable Focus Beam	✓
Fluorescence data collection	✓
Tuneable MAD	✓
Interleaved energy data collection
Inverse beam data collection
Multi-axis goniometry	✓
IMISX Capable *for LCP in situ* studies - discuss with staff before arrival**	✓
In situ goniometry for crystallisation plate	✗
Automated strategy calculation	✓
Automated data processing	✓
Automated difference map calculation	✓
Automated phasing	✓
Automated molecular replacement	✓
Remote Access	✓

I04 Specifications

Standard energy (Å / keV)	0.9537 / 13.00
Flux at 13.000 keV (ph/s)	1.25 x 10¹²
Wavelength range (Å)	0.69 - 2.07
Energy range (keV)	6.0 - 18.0
Beam size at sample (µm)	10 x 5 --> 110 x 100 (h x v)
Detector	Eiger2 XE 16M
Time to collect 180^o data set (seconds)	3.2 ⁺
Sample Changer	BART
No. of pins in dewar	592
Sample exchange time (seconds)	18
Guide to samples screened/hour (user centering)	30

⁺ 0.1^o per 0.0018 second exposure 1800 frames (shutterless)

Features include:

Variable focus beam from 10 x 5 to 110 x 100 µm delivering 6.7 x 10¹¹ ph/s at the Se K edge and 1.25 x 10¹²ph/s at 13.0 keV (20 micron beam size at 300 mA ring current)
Dectris Eiger2 XE 16M detector
Smargon multi-axis goniometer
High resolution viewing system
Accurate sample centering stages
Simple beam visualisation procedures
Fine resolution attenuation control
DLS BART sample changer - 37 Unipucks / 592 samples
Vortex fluorescence detector
Automated data processing
Remote access

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I04 Contact

Science Group Leader

Dave Hall

I04 Microfocus MX