Fixed target

Pipette chip loading for fixed target needs about 150 µL of crystal slurry with a density of about 10⁶ - 10⁷ crystals / mL. In order to reduce the amount of sample needed, we integrated the picolitre dispenser PolyPico with the same stages used on I24 to raster the chip at the beamline. We can can load a full chip with as little as 3-5 µL with a crystal density of 10⁷ - 10⁸ crystals / mL in about 6 min (Figure 1).

Figure 1 (from Davy et al., 2019). Experimental setup for (a) calibration of ejected droplets and (b) chip loading; in each, the directionof droplet ejection is shown in blue. (c) Schematic of chip loading from a similar viewpoint to (b)with translation stages hidden and the cartridge highlighted in yellow.

For more details please see our paper (Davy et al., J Synchrotron Radiat. 2019) and feel free to contact us.