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Why choose Cryo-soft X-ray Tomography (cryo-SXT) at Diamond for your research?

Using cryo-Soft X-ray Tomography (cryo-SXT) at Diamond presents cutting-edge capabilities to visualise cellular architecture with outstanding 25nm resolution. This method preserves the native state of your samples, allowing you to investigate intricate molecular interactions within whole cells to observe biological processes - from viral replication to sporulation—in unprecedented detail.

The unique setup of the facility enables a correlative microscopy approach to your research, combining X-ray tomography with high-resolution fluorescence microscopy in the same location. This powerful combination not only captures the intricate dance of molecular interactions but also pinpoints biological structures within the cell, offering unparalleled insight into the inner workings of life.

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Benefits of using cryo-soft X-ray Tomography at Diamond

  • Enables users to study the ultrastructure of vitrified whole cells
  • Provides the capability to capture cellular images to a resolution of 25-40 nanometres.
  • Allows investigations of molecular interactions in cellular environments
  • Enables users to study cells at near-native states (no fixation, staining or micro-sectioning required)
  • Facilitates the study of relatively thick biological samples
  • Allows longer exposures and serial imaging without significant radiation-induced sample deterioration.
  • Integrates with complementary techniques such as cryo-EM and light microscopy modalities.

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