B24 is the full-field transmission microscope at Diamond, designed specifically to meet the rising demand for tomographic imaging of biological specimens under near physiological conditions. This technique bridges the resolution gap between electron microscopy and conventional light microscopy, allowing the acquisition of tomographic data from both native and fluorescent-labelled samples.
Benefits:
- Image ultra structures: Capture detailed images of whole cells and cell populations.
- Native state imaging: Image vitrified cells in their native state without chemical or physical preservation.
- Fast and accurate: Benefit from automatic data processing for quick and precise results.
- Monitor cellular changes: Observe the effects of infection or pharmaceutical dosing on cells.
- Optimal data collection: Collect data at ~500eV (water window) using absorption contrast mode.
- Extensive field of view: Utilise a 16 x 16 micron field of view with stitching capability.
- High resolution: Achieve resolutions of ~25-40nm in samples up to ~12µm thick.
- Minimal sample processing: Work with vitrified samples that are not sectioned, stained, dehydrated, or otherwise processed.
- Automated processing: Enjoy the convenience of automatic data processing.
Best For:
- Near Physiological Conditions: Ideal for imaging biological specimens in conditions that closely mimic their natural state, providing more accurate and relevant data.
- Whole Cells and Cell Populations: Perfect for capturing detailed images of entire cells and groups of cells, allowing for comprehensive analysis of cellular structures and interactions.
- Monitoring Cellular Changes: Highly effective for observing the effects of various treatments, such as infections or pharmaceutical dosing, on cellular structures and functions.